Xenbase Image ID: 84397

Fig. 6. Overexpression of either X. tropicalis XLPA1 or XLPA2 mRNA causes an increase in cortical actin and more rapid wound healing, mimicking LPA treatment. (A) The bases (after excision of animal caps) of embryos injected with 400 pg of XLPA1 elongated along the animal-vegetal axis after the vitelline membrane was removed and were more rigid than control embryos. These bases healed faster than controls. Scale bar: 350 μm. (B) Bases from embryos injected with XLPA2 mRNA showed a similar phenotype as bases from XLPA1-injected embryos. Scale bar: 450 μm. (C) Animal caps from XLPA2-injected embryos rounded up faster than control caps after 10 minutes of healing. Scale bar: 125 μm. Injection of either XLPA1 (D) or XLPA2 (E) mRNA increased the levels of cortical actin in the animal cap cells and in the purse-string compared with controls. Scale bar: 50 μm. Image published in: Lloyd B et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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