Xenbase Image ID: 126040

Figure 4. Validation of the identification of hnRNP G and inter-species conservation of hnRNP G interaction with the WEc RNA. 1D-NWA of the binding of the 32P-labelled RNA WEc probe with nuclear proteins extracts from different species, followed by immunodetection using the canine anti-hnRNP G serum. (A) hnRNPG homologues in ZZ and ZW GVs of P. waltl. The 42 kDa labelled band (box) detected by NWA in ZW GVs corresponded exactly to the one detected by the anti-hnRNP G serum. Note that a band corresponding to hnRNP G was immunodetected in ZZ GVs, although no signal was visible in the corresponding blot after NWA. (B) hnRNP G from nuclear proteins extracts of human HeLa cells and GVs of the amphibians X. topicalis (X.t), N. viridescens (N.v) A. mexicanum (A.m) and P. waltl (P.w, ZW karyotype). Stars and circles indicate the bound hnRNP G. Image published in: Kanhoush R et al. (2011) © The Author(s) 2011. Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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