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Figure 8. SERCA activity regulates Aβ production. (A) SERCA2b overexpression leads to higher Aβ levels. Aβ40 and Aβ42 levels were measured by sandwich ELISA. Note that Aβ40 levels are significantly increased in SERCA2b-transfected cells, compared with control pcDNA vector-treated cells (*, P < 0.05; n = 5). (B) Genetic down-regulation of SERCA2b expression lowers Aβ levels. siRNA-mediated down-regulation of SERCA2b leads to lower steady-state Aβ levels when compared with cells treated with a control (scrambled) siRNA (*, P < 0.05; n = 6). (C and D) Pharmacological inhibition of SERCA activity lowers steady-state Aβ levels. Inhibition of SERCA2b with 1 μM thapsigargin in the presence of extracellular Ca2+ decreases Aβ40 levels within 4.5 h (n = 4) and also decreases Aβ42 levels within 11.5 h (n = 4). 4.5 h: *, P < 0.005 for thapsigargin and control; *, P < 0.05 for thapsigargin and DMSO. 11.5 h: *, P < 0.005 for thapsigargin and control; *, P < 0.05 for thapsigargin and DMSO. Error bars show SEM.

Image published in: Green KN et al. (2008)

© 2008 Green et al. Creative Commons Attribution-NonCommercial-ShareAlike license

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