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Figure 18. (A) The fractional changes in conductance and rectification ratio were correlated. Data points include charge changes brought about by thiol modification of R334C CFTR with positively charged reagents (open triangles), and negatively charged reagents (closed triangles), pH titration of R334C CFTR (closed circles), and pH titration of R334H CFTR (open circles). The solid line is the least-squares fit to the data. (B) Fractional change in conductance plotted versus the fractional change in elementary charge at position 334. Thiol modification of R334C CFTR with positively charged reagents (open triangles) was treated as adding a single positive charge; thiol modification of R334C with negatively charged reagents (closed triangles) was treated as adding a single negative charge; pH titration of R334H CFTR (open circles) was treated as adding a time-average positive charge determined by the bath pH, assuming a pKa of 5.68; and pH titration of R334C CFTR (closed circles) was treated as adding a time-average negative charge determined by the bath pH assuming a pKa of 8.17. Solid line is least-squares fit to the data, dashed lines are 95% confidence limits. Image published in: Smith SS et al. (2001) © 2001 The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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