XB-IMG-124006
Xenbase Image ID: 124006
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Figure 3. Co-expression of NALCN and M3R in the HEK-293 cell line and in Xenopus oocytes results in the expression of an M3R agonist-activated current. (A) Co-expression of NALCN and M3R cDNAs in HEK-293 cells results in the expression of an acetylcholine (ACh)-activated inward current with the same profile as in the MIN6 cells. (B) Mean acetylcholine-activated current density in HEK-293 cells expressing NALCN and M3R. (C) Co-expression of NALCN and an M3R deletion mutant (M3R*) that is deficient in the activation of G proteins in Xenopus oocytes results in the expression of a carbachol (CCh)-activated inward cationic current. (D) Mean carbachol-activated current density in Xenopus oocytes, mock-injected or expressing M3R, NALCN, M3R* or NALCN+M3R*. (E) Leak activity in HEK-293 cells and (F) in Xenopus oocytes is not significantly altered by NALCN overexpression. cDNA, complementary DNA; HEK, human embryonic kidney; M3R, M3 muscarinic receptor; NALCN, sodium leak channel, non-selective. Image published in: Swayne LA et al. (2009) Copyright © 2009, European Molecular Biology Organization. Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View |