Xenbase Image ID: 146934

Fig. 4. Analysis of genes regulating brain patterning in XTsh1a-injected embryos and XTsh1 morphants. (A, B) XWnt-4 expression is unchanged in XTsh1a-injected embryos but repressed in XTsh1 morphants. (C, D) FGF-8 expression remains unaffected. (E–G) XMeis3 expression is unchanged in XTsh1a-injected embryos but shifts posteriorly in XTsh1 morphants. A–F: albino embryos of stage 16 were used for in situ hybridization, G: wild-type embryo of stage 19 is shown. Embryos were injected with XTsh1a mRNA (500 pg) or XTsh1-MO (2.5 pmol) into one of two cells. Fluorescein dextran was used as lineage tracer. is: injected side, nis: non-injected side. Anterior pole of the embryos is orientated to the upper edge of the frame. Image published in: Koebernick K et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + tshz1 MO + NF16 (in situ hybridization) Fig 4 F Expression Phenotype mislocalised meis3.L expression in chordal neural plate Xla Wt + tshz1 MO + NF16 (in situ hybridization) Fig 4 B Expression Phenotype decreased amount wnt4.L expression in neural tube decreased amount wnt4.L expression in presumptive midbrain-hindbrain boundary Xla Wt + tshz1 MO + NF16 (in situ hybridization) Fig 4 F Expression Phenotype mislocalised meis3.L expression in lateral plate mesoderm Larger Image Printer Friendly View

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