Xenbase Image ID: 121290

Figure 6. . The β1 subunit greatly increases burst duration and Po, and leftward shifts V0.5 after the Ca2+ bowl mutation D965N to the Slo1 channel. (A) Representative single-channel currents recorded from Slo1 channels, and Slo1 channels with the Ca2+ bowl mutation D965N without, and with β1 subunits. 20 μM Ca2+i, 30 mV. (B) Representative macrocurrents recorded from the D965N mutant Slo1 channels with and without β1 subunits. The potential was held at −120 mV, stepped to potentials between −100 to 200 mV in increments of 10 mV, and then stepped to −80 mV to measure tail currents. (C) G-V curves derived from tail currents of Slo1 channels, D965N mutant Slo1 channels, and D965N mutant Slo1 channels with β1 subunits (20 μM Ca2+i). The average voltage sensitivity was 19.1 ± 0.6 mV/e-fold without the β1 subunit and 23.1 ± 1.2 mV/e-fold with the β1 (n = 5 patches in each case except 3 patches for Slo1). (D) Plots of V0.5 for Slo1 channels, and D965N mutant Slo1 channels without and with β1 subunits. Image published in: Qian X et al. (2002) Copyright © 2002, The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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