XB-IMG-125271
Xenbase Image ID: 125271
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Figure 6. KHDC1 proteins associate with mCPEB1. (A) Flag-KHDC1A and Flag-KHDC1B coimmunoprecipitate with HA-mCPEB1 expressed in Xenopus embryos or in 293T cells. (B) The interaction between KHDC1B and mCPEB1 is independent of RNA. (C) Full-length mCPEB1 and the deletion constructs used to test for interaction with KHDC1B. The PEST domain is a motif that regulates protein stability. The RRM (RNA recognition motif) and ZF (zinc finger) domains control RNA binding. (D) HA-mCPEB1 and HA-mCPEB1-Cter coimmunoprecipitate with Flag-KHDC1B when coexpressed in 293T cells. HA-CPEB-Nter did not interact with KHDC1B in this assay. * in the input indicates nonspecific signal. For input, 5% of the total lysate was loaded. Image published in: Cai C et al. (2010) © 2010 by The American Society for Cell Biology. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |