Xenbase Image ID: 197116

Figure 4. Effects of MB on K+ currents mediated through the C-terminally deleted Kir6.2 channels (KirΔC26) and the effects of cGMP modulating agents on MB inhibition of cromakalim-activated K+ currents. (A) Currents from inside-out patches in response to two s-voltage-ramps from –100 to +100 mV under symmetric conditions (140 mM K+) were recorded in the absence and presence of 30 μM MB. Leak currents, recorded in the presence of 10 mM ATP, were subtracted. (B) Time-course of the effect of 30 µM MB on K+ currents mediated through the C-terminally deleted Kir6.2 channels (KirΔC26) in the absence (filled circles) and presence (open circles) of MB (30 μM), respectively. The horizontal bar represents the administration time of MB. (C) The effects of 20 min preincubation with BAY 58–2667 (3 µM) and 8-Br-cGMP (100 µM) on inhibition of cromakalim (100 μM)-activated K+ currents by MB (30 µM) in follicle-enclosed oocytes (p > 0.05, n = 5–7, Student’s t-test). MB: methylene blue; Bay 58: BAY 58–2667. Image published in: Isaev D et al. (2023) Copyright © 2023. Image reproduced with permission of the Publisher. Larger Image Printer Friendly View

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