XB-IMG-125508
Xenbase Image ID: 125508
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Figure 6. Gemcitabine induces hypermethylation and silencing of MLH1.(A) Methylation sensitive PCR analyzing MLH1 promoter methylation state in MCF7 or HEK293 cells. Cells were treated as indicated with increasing concentrations of gemcitabine (Gem, 33–134 nM) or etoposide (Eto, 43 nM) as control. HpaII restriction is methylation sensitive and allows the quantification of the MLH1 methylation state. As control, samples were also treated with the methylation insensitive isoschizomer MspI. Error bars represent the standard deviation of three biological replicates. Significance was assessed via unpaired Student's t-test using the untreated sample as reference: * = p<0.05; ** = p<0.01. (B) Cells were treated as in (A). Relative expression of MLH1 normalized to GAPDH was monitored by qPCR. Error bars represent the standard deviation of three biological replicates. Significance was assessed via unpaired Student's t-test using the untreated sample as reference: * = p<0.05; ** = p<0.01; *** = p<0.001. Image published in: Schäfer A et al. (2010) Schäfer et al. Creative Commons Attribution license Larger Image Printer Friendly View |