Xenbase Image ID: 121092

Figure 6. Protein expression of CFTR constructs severed near the NH2 terminus of NBD1. (A) Immunoblot of membrane proteins from oocytes expressing individual CFTR segments or pairs of segments, as indicated, blotted with anti–R-domain antibody. (B) Coexpressed constructs Flag3-432+433-1480 were digested with N-glycosidase-F (left) and endoglycosidase-H (right), and the products were identified with anti–R-domain antibody. Arrows in A and B mark fully glycosylated (∼160 kD, fat arrow), core glycosylated (∼125 kD, thin arrow), and deglycosylated (∼120 kD, thin arrow) forms of 433-1480 protein. *Partially degraded form (∼70 kD). Image published in: Chan KW et al. (2000) © 2000 The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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