Xenbase Image ID: 122336

Figure 3. . Cysteine substitutions of S4's positive charges had little effect on the inward rectification property and reversal potential of hERG. (A) Original current traces were elicited by the voltage clamp protocol diagrammed on top. From Vh −80 or −90 mV, a strong depolarization pulse to +60 mV for 0.2 s was used to activate and inactivate the channels. This was followed by repolarization pulses from +30 to −120 mV in 10-mV increments (current traces shown were from +20 to −120 mV in 20-mV steps) during which channels rapidly recovered from inactivation and then deactivated. Recordings were made in 2 mM [K]o (estimated EK −105 mV, assuming [K]i 125 mM). (B) Current–voltage relationships from experiments as shown in A. The peak or plateau amplitudes of tail currents were normalized by the maximum outward tail current in each cell (occurring at Vr −50 to −30 mV) and averaged. Gray open circles and black circles are for WT and mutant, respectively (n = 6–14 each). Image published in: Zhang M et al. (2004) Copyright © 2004, The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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