Xenbase Image ID: 123349

Figure 4. Specificity of Human SLC2A9a and SLC2A9b Antibodies and Urate Uptake into SLC2A9 Transfected Human Embryonic Kidney Cells(A) Western blotting of hSLC2A9 expressed in HEK cells. Expression of hSLC2A9a was detected as a broad band at approximately 50 kDa by a polyclonal antibody raised against the N terminus of SLC2A9a in HEK293 cells overexpressing SLC2A9a (middle lane, Slc2a9a) but not detected in cells overexpressing SLC2A9b (right lane, Slc2a9b), or nontransfected HEK cells (left lane, HEK).(B) Expression of hSLC2A9b in transfected HEK293 cells. hSLC2A9b was detected by a polyclonal antibody raised against the N terminus of hSLC2A9b in HEK293 cells overexpressing hSLC2A9b (right lane, Slc2a9b), but not detected in cells overexpressing hSLC2A9a (middle lane, Slc2a9a).(C) Increase in urate uptake in HEK293 cells overexpressing either hSLC2A9a or hSLC2A9b. Radiolabelled urate uptake was measured in human embryonic kidney cells, which were stably overexpressing SLC2A9a or SLC2A9b as compared to their respective nontransfected controls. Uptakes of 120 μM urate were measured over 6 min at 37 °C. *p < 0.05; **p < 0.02. Image published in: Caulfield MJ et al. (2008) Copyright: © 2008 Caulfield et al. Creative Commons Attribution license Larger Image Printer Friendly View

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