Xenbase Image ID: 122028

Figure 13. Effect of [Cl]i on the block. [Cl]i was changed from 104 mM (standard solution) to 14 mM (Cl− was replaced by glutamate) on inside-out patches with no or with 1 mM S(−)-CPB present in the intracellular solution. (A) The effect of this maneuver on the apparent open probability in the absence of CPB. The parameters of the fits () are as follows: for high [Cl]i, pmin = 0.16, V1/2 = −90 mV, and z = 0.98; low [Cl]i: pmin = 0.04, V1/2 = −57.8 mV, and z = 0.94. Using the analysis described for normal [Cl] the apparent Kd (B) and the effective on (C) and off (D) rate constants were determined as a function of voltage. The dashed line in C was obtained in the following way: an arbitrary function was fitted to the data in high [Cl]i and the same function was replotted, multiplied by a factor of five. Solid lines in C and D were calculated from a simulation of Fig. 4. The parameters were as in Table . Image published in: Pusch M et al. (2001) © 2001 The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

Return to previous page