XB-IMG-127121
Xenbase Image ID: 127121
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Figure 5. Characterization of the 5-HT3A-A1 insertion construct.(A) Currents elicited by repeated applications of 10 µM 5-HT are significantly smaller than the current following the initial 5-HT application in the 5-HT3A-A1 receptor. The wash time denoted by the//was 6 min. Insert shows an expanded time scale of the indicated region. The second current response amplitude was 11.6% of the initial response. (B) Radioactive 5-HT binding to oocytes expressing 5-HT3A-WT and 5-HT3A-An insertion constructs was determined by subtracting 5-HT binding to water-injected oocytes from 5-HT binding to channel expressing oocytes. Each bar represents the mean 5-HT binding ± SEM for at least 10 channel expressing oocytes from at least three separate oocyte isolations. ** indicates radioactive 5-HT binding significantly different from WT by one way ANOVA using Dunnett's multiple comparison post hoc test (p<0.0001). Image published in: McKinnon NK et al. (2012) McKinnon et al. Creative Commons Attribution license Larger Image Printer Friendly View |