Xenbase Image ID: 84752

Fig. 2. Amphibian PouV proteins can support mouse ES cell self-renewal. (A) The indicated PouV cDNAs were co-transfected with the Fgf4 enhancer or the octamer-binding motif luciferase reporters. Fold induction represents the increase in transcription compared with the vector-only control. Experiments were carried out in triplicate. (B) Morphology of day 9 puromycin resistant colonies transfected with the indicated cDNA in the presence or absence of Oct4 transgene expression. Colonies are stained for AP activity (red). Scale bars: 100 μm. (C) Rescue index for PouV proteins. The rescue index=the number of ES cell colonies in the absence of Oct4 /number of ES cell colonies in the presence of Oct4. Mouse Oct4 was assigned a value of 1 to which all other values were normalized. Data represents the mean values obtained from three independent experiments. Image published in: Morrison GM and Brickman JM (2006) Copyright © 2006. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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