XB-IMG-123404
Xenbase Image ID: 123404
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Figure 1. Pan-Neuronal Expression of Membrane-Tethered Spider Toxins Induces Lethality in Transgenic DrosophilaFlies homozygous for an elav-GAL4 transgene were mated to flies heterozygous for the indicated UAS-toxin transgenes and a phenotypically marked balancer chromosome, such that one half of the progeny receive both elav-GAL4 and UAS-toxin transgenes, while the other half of the progeny receive elav-GAL4 and the balancer chromosome. Lethality was assessed as percent relative mortality, which is 100% if there are no viable adult flies with both transgenes, and 0% if there are the same number of viable adult flies with both transgenes as with elav-GAL4 and the balancer. Negative mortality reflects a fitness disadvantage of progeny carrying a balancer chromosome relative to progeny carrying a chromosome with a UAS-toxin transgene insertion. The code numbers refer to independent chromosomal insertions of the indicated UAS-toxin transgenes. Code numbers 7.4a and 7.6a indicate two independent insertions of a membrane-tethered μO-MrVIA UAS transgene, which encodes a vertebrate Na+ channel blocker with no activity against Drosophila ion channels. Pan-neuronal expression of membrane-tethered μO-MrVIA has no effect on viability of flies. However, pan-neuronal expression of either one of the four indicated spider toxins induces complete embryonic/larval lethality for all independent UAS-toxin chromosomal insertions tested. n > 50 animals for each cross. Image published in: Wu Y et al. (2008) Copyright: © 2008 Wu et al. Creative Commons Attribution license Larger Image Printer Friendly View |