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Figure 4. Cell death and UNC-8(d) permeability properties. (A) Representative photographs of a noninjected oocyte (top), oocyte-injected UNC-8 wild type, and an UNC-8(G387E) (middle and bottom panels, respectively) incubated for 24 h in OR2 solution containing 5 µM calcium and no magnesium. Note the lysis of the oocyte-expressing UNC-8(G387E). (B) Quantification of the ratio of oocytes that are intact after a 24-h treatment in OR2 containing 5 µM calcium. Values are averages of four independent injections with 10 oocytes in each replicate. Amiloride was added to a final concentration of 500 µM. Values are mean ± SE. **, P ≤ 0.01 by ANOVA. (C) Example of currents recorded in a noninjected oocyte (top) and an oocyte injected with UNC-8(G387E) perfused with a solution whose only permeant cation was calcium. Voltage steps were from −160 to −40 mV from a holding potential of 0 mV. (D) Average current amplitudes at −160 recorded from noninjected oocytes and oocytes injected with UNC-8(G387E) perfused with the calcium solution. Note that the oocyte-endogenous Ca2+-activated Cl− current is not activated in either sample (Bianchi et al., 2004). Data are expressed as mean ± SE (n = 8). NS, not statistically different. (E) Average current amplitude recorded at −100 mV in oocytes injected with UNC-8(G387E) perfused the solutions indicated on the x axis. Data are expressed as mean ± SE (n = 12, 12, 12, and 9, respectively). Image published in: Wang Y et al. (2013) © 2013 Wang et al. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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