Xenbase Image ID: 116934

Figure 1. 4-AP blocks Shaker only after channel opening, and it can be trapped by channel closure. Using a fast-perfusion system, 4-AP (3 mM) was applied intracellularly to an inside-out patch expressing Shaker channels. (A) Blocker was applied during a long depolarizing pulse to +60 mV and removed before returning to a holding potential of −90 mV. (B) 4-AP was perfused during a depolarizing pulse and washed out immediately after returning to a membrane potential of −90 mV. After a 2-min wash out at −90 mV, a new depolarizing step to +60 mV was applied. (C) blocker was applied for over 1 s at −90 mV and removed immediately before channel opening. Image published in: del Camino D et al. (2005) Copyright © 2005, The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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