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XB-IMG-121739

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Figure 3. Slowing of deactivation as a function of activation step duration is not unique to α + β3b currents. In A1–D1, outward current was activated with 10 μM Ca2+ by a step to +100 mV from a holding potential of −120 mV. For each sweep, the step to +100 mV was varied from 0 to 9.5 ms in 0.5-ms increments, before a repolarizing step to −80 mV. Currents resulted from the following constructs: (A) α alone; (B) α + β3b-ΔN; (C) α + β3b; and (D) α + β3b-ΔC. For these constructs, a direct step to −80 mV from −120 mV results in minimal inward current activation. A2–D2 show the tail currents on an expanded time base. For α alone (A2) and for α + β3-ΔN (B2), tail currents become slower with command step duration, but the decay qualitatively appears to begin a single exponential time course immediately after the repolarizing step. For α + β3b (C) and α + β3b-ΔC (D), tail current decay also appears to slow with command step duration. However, in contrast to the noninactivating currents, as the command step is increased in duration there is an increase in a shoulder of tail current that precedes the onset of an exponentially decaying current. For all panels, a P/N leak subtraction procedure was used (see materials and methods).

Image published in: Lingle CJ et al. (2001)

© 2001 The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license

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