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Fig. 2. The N-termini P2X1 receptor minigene blocks the potentiating effects of PMA and mGluR1α receptor stimulation on P2X1 receptor currents. A minigene encoding the N-terminal sequence of the P2X1 receptor was co-expressed with wild type P2X1 and mGluR1α receptors in the Xenopus oocytes. (a) Upper left panels show representative currents evoked by a maximal concentration of ATP (100 μM, indicated by bar) at control oocytes (WT P2X1) and those following 10 min incubation with PMA (100 nM). Right upper panels show the effects of co-expression of the amino terminal minigene (NH2 minigene) on the effects of PMA. The bar chart shows summary data, n=6–7. (b) Upper panels show sample traces for a given oocyte co-expressing P2X1 and mGluR1α receptors (left) or P2X1 receptors, mGluR1α receptors and the P2X1 receptor amino terminal minigene (right traces). Responses to a maximal concentration of ATP (100 μM, indicated by bar) are shown before and after the application of glutamate (100 μM). Glutamate evoked an inward calcium activated chloride current and potentiated subsequent ATP evoked responses. This potentiation was reduced by co-expression of the P2X1 receptor N-terminal minigene. The bar chart shows a summary of the data, n=6–7. ***p< 0.001.

Image published in: Wen H and Evans RJ (2009)

Journal compilation © 2009 International Society for Neurochemistry. Creative Commons Attribution license

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