Xenbase Image ID: 122717

Figure 3. C22 aldehyde is a potent inhibitor of the homomeric rod CNG channel. (A) The raw traces represent families of cGMP-activated currents in response to voltage steps ranging from −100 to +100 mV in 50-mV increments from a holding potential of 0 mV. Currents measured in the absence of cGMP were subtracted from all traces. Black traces represent currents in saturating cGMP before the addition of C22 aldehyde; red traces represent currents after 1 h (steady state) in 400 nM C22 aldehyde (46% inhibition). (B) The dose–response relation for C22 aldehyde inhibition of the rod CNG channel. All points were measured at +100 mV after 1 h (steady state) in the indicated concentration of C22 aldehyde and saturating (2 mM) cGMP. Data were normalized to maximal current in 2 mM cGMP in the absence of C22 aldehyde, with leak current in the absence of cGMP subtracted from each trace. Each point is an average from two to three patches, and the smooth curve is a fit with the Hill equation, where the IC50 = 415 nM, and n = 2.5. Image published in: Horrigan DM et al. (2005) Copyright © 2005, The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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