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Fig. 3. Focal adhesion dynamics and actin cytoskeleton are altered in krt8 morphant explants. (A,C,E,G) Representative TIRF images of mesendoderm explants expressing LifeAct- mCherry (red) and EGFP-paxillin (green). (B-B′′,D- D′′,F-F′′,H-H′′) Magnified views of the boxes in A, C, E and G, corresponding to merged (B,D,F,H) and separate LifeAct-mCherry (B′,D′,F′,H′) and EGFP- paxillin (B′′,D′′,F′′,H′′) channels. (I-K) Quantifications of FA area (I), FAs per cell (J) and distribution of FAs within cells (K), calculated from the first frames of nine movies for each condition (Movies 3-6) (nine individual explants per condition were imaged across three separate experiments; data are mean±s.e.m. *P<0.05; **P<0.01; ***P<0.001; ns, not significant). Scale bars: 25 μm in A, C, E and G; 10 μm in B, D, F and H.

Image published in: Sonavane PR et al. (2017)

Copyright © 2017. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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