Xenbase Image ID: 124846

Figure 3. Effect of shRNA structure on RNAi efficacy. (A) Diagram of the pSi constructs containing shGFP or shGFPmir30 with or without Ago2 co-expression (left), and the pRe construct (right) for simultaneous GFP and mCherry expression. (B) G/R fluorescence intensity ratio measured from cells transfected with pSi constructs containing shGFP or shGFPmir30 with or without Ago2 co-expression. For shGFPs, Ago2 co-expression broadly enhances GFP knockdown (red bars). For shGFPsmir30, the RNAi efficiency increases for shGFP-1mir30 and shGFP-2mir30 but not shGFP-3mir30 (yellow bars). Additionally, Ago2 co-expression enhances knockdown with all shGFPsmir30to a level comparable to Ago2 co-expression with shGFPs. Asterisks represent statistical significance (p < 0.05, ANOVA-Scheffe's test). Image published in: Chen CM et al. (2009) Image downloaded from an Open Access article in PubMed Central. Copyright © 2009 Chen, Chiu, Shen and Cline. Larger Image Printer Friendly View

Return to previous page