XB-IMG-122757
Xenbase Image ID: 122757
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Figure 1. ELYS/MEL-28 Is a Chromatin-Associated Protein(A) Chromatin was isolated from egg extract at discrete time points throughout interphase and subjected to LC-MS/MS. The relative abundance of ELYS/MEL-28, polymerase δ, Mcm2, and Nup153 are indicated. The duration of S phase in this extract is indicated above the graph.(B) Sperm chromatin was incubated in either a metaphase or interphase egg extract. At the indicated times, chromatin was isolated and immunoblotted for Mcm2, SMC2, and ELYS. As a control, extract was incubated without sperm chromatin for 90 min. The lower portion of the gel was stained with Coomassie blue so that the level of histones could be determined as a control for chromatin recovery. Image published in: Gillespie PJ et al. (2007) © 2007 ELL & Excerpta Medica. Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View |