Xenbase Image ID: 129533

Figure 2. Glucose transport activity of C. elegans GLUT-like transporters expressed in Xenopus oocytes(A) The levels of GLUT-like transporters at the cell surface as determined using exofacial HA epitopes. cRNAs for transporters HA-tagged in the first exofacial loop were injected into oocytes. The HA tag at the cell surface was detected with an anti-HA antibody and a β-galactosidase-conjugated secondary antibody. Results are means±S.E.M. for three separate experiments. *P<0.05 compared with the water-injected control oocytes. (B) Glucose-transport activity associated with the indicated putative GLUT-like transporters (without HA tags). Results are means±S.E.M. for three separate experiments. ns, not significantly different compared with the water-injected control oocytes. (C) Comparison of glucose-transport activities, in the presence and absence of the HA tags, that are associated with the expression of hGLUT1 and C. elegans FGT-1A and FGT-1B. Results are means±S.E.M. for three separate experiments. *P<0.05 compared with the water-injected control oocytes. (D) Uptake of 50 μM D-glucose via FGT-1A expressed in oocytes. Time courses were found to be linear. Results are means±S.E.M. for three separate experiments. (E) Rates of maximal transport for glucose in Xenopus oocytes expressing 50 ng of cRNA for FGT-1A and FGT-1B. Oocytes were incubated with the indicated concentrations of D-glucose (S). The rates of [14C]glucose uptake (v, pmol glucose/oocyte per min) were used in least-square fits to the Michaelis–Menten equation. Results are means±S.E.M. for three separate experiments. Image published in: Feng Y et al. (2013) © 2013 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons. Creative Commons Attribution license Larger Image Printer Friendly View

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