Xenbase Image ID: 147087

Figure 1. Expression pattern of Hspa9 in Xenopus laevis embryo. A: RT-PCR hspa9 expression in Xenopus laevis embryos from stages 3 to 40 of development with odc1 as house-keeping gene. B–H: Whole-mount ISH of hspa9. B: Early gastrula, arrow indicates the position of the blastoporal lip. C: Early neurula stage (anterior view, dorsal side up) shows hspa9 expression in neural and mesodermal territories. D,E: hspa9 is expressed in somite (s), mesoderm (m) and intermediate mesoderm (im). F: hspa9 labels the pronephros anlage (pa). G: At early tail bud stage, strong expression of hspa9 in eye (e), otic vesicle (ov), branchial arches (ba), somites and pronephros, from proximal (red arrow) to distal part (black arrow). G′: Section through the plan of dotted line in G. H,H′: Tadpole stage shows persistent hspa9 expression in the pronephros tubule. I–L: hspa9 pronephros expression was compared with pax8 and pax2 renal markers. Arrows show similar hspa9 and pax expression domains, up to the tip of the nephric duct. A strong hspa9 expression is present in the proctodeum (asterisk in I, K). Abbreviations: dt, distal tubule; g, glomus; it, intermediate tubule; n, notochord; pt, proximal tubule. Scale bars = 0.5 mm in B–H; 1 mm in I–L.Download figure to PowerPoint Image published in: Gassié L et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue hspa9.L csa, grp-75, grp75, hspa9-a, hspa9-b, hspa9b, mortalin, mot, mot2, pbp74 X. laevis Throughout NF stage 10 mesoderm involuted dorsal mesoderm neuroectoderm hspa9.L csa, grp-75, grp75, hspa9-a, hspa9-b, hspa9b, mortalin, mot, mot2, pbp74 X. laevis Throughout NF stage 14 mesoderm anterior dorsal lateral plate region dorsal lateral plate mesoderm neural fold anterior neural fold hspa9.L csa, grp-75, grp75, hspa9-a, hspa9-b, hspa9b, mortalin, mot, mot2, pbp74 X. laevis Throughout NF stage 18 somite mesoderm hspa9.L csa, grp-75, grp75, hspa9-a, hspa9-b, hspa9b, mortalin, mot, mot2, pbp74 X. laevis Throughout NF stage 20 mesoderm somite intermediate mesoderm hspa9.L csa, grp-75, grp75, hspa9-a, hspa9-b, hspa9b, mortalin, mot, mot2, pbp74 X. laevis Throughout NF stage 22 mesoderm somite intermediate mesoderm pronephric mesenchyme anterior dorsal lateral plate region hspa9.L csa, grp-75, grp75, hspa9-a, hspa9-b, hspa9b, mortalin, mot, mot2, pbp74 X. laevis Throughout NF stage 24 optic vesicle cranial neural crest mandibular crest hyoid crest midbrain-hindbrain boundary pronephric mesenchyme mesoderm proctodeum hindgut pax8.L pax-8, XPax-8, XPax8 X. laevis Throughout NF stage 24 otic placode pronephric mesenchyme lateral plate mesoderm hspa9.L csa, grp-75, grp75, hspa9-a, hspa9-b, hspa9b, mortalin, mot, mot2, pbp74 X. laevis Throughout NF stage 31 eye otic vesicle branchial arch somite head somite trunk somite pronephric kidney pronephric duct glomus pronephric nephron pronephric nephrostome hspa9.L csa, grp-75, grp75, hspa9-a, hspa9-b, hspa9b, mortalin, mot, mot2, pbp74 X. laevis Throughout NF stage 35 and 36 head mesoderm midbrain-hindbrain boundary somite head somite trunk somite tail somite otic vesicle proctodeum pronephric kidney pronephric duct glomus pax2.L LOC108697493, pax-2, pax2-a, pax2-b, XPax-2, XPax2 X. laevis Throughout NF stage 35 and 36 lateral plate mesoderm dorsal lateral plate mesoderm pronephric kidney pronephric nephrostome pronephric duct pronephric nephron glomus hindbrain head mesenchyme hspa9.L csa, grp-75, grp75, hspa9-a, hspa9-b, hspa9b, mortalin, mot, mot2, pbp74 X. laevis Throughout NF stage 37 and 38 eye retina lens head mesoderm otic vesicle midbrain-hindbrain boundary forebrain-midbrain boundary branchial arch somite head somite trunk somite pronephric kidney pronephric nephrostome pronephric duct pronephric nephron glomus pronephric tubule proximal tubule distal tubule early distal tubule Image source: Published Larger Image Printer Friendly View

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