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Figure 5âfigure supplement 1.
Effects of Six1 knockdown on target genes.
Tail bud stage (AâG) and neural plate stage (HâJ) embryos after unilateral injection of Six1-MO1+2. In each case, lacZ was co-injected as a lineage tracer and panels A1âG1 show the control (un-injected) side and A2âG2 show the injected side. The injected side is positioned to the right in HâJ, as marked by blue lacZ staining (lacZ staining out of frame in some specimens). Arrows mark reductions in marker gene expression in placodal derivatives, and asterisks indicate increased expression.