Xenbase Image ID: 158073

Figure 1 β-Trcp forms a complex with β-catenin and Axin in vivo. (A and B) Association between β-Trcp and β-catenin. (A) Immunoprecipitation of β-Trcpmyc with an anti-myc antibody. Embryo extracts were from stage 9 embryos expressing β-catenin, β-catenin (S→A), or β-Trcpmyc alone or in combination. The precipitates were examined for β-catenin (Upper) and for β-Trcpmyc (Lower). A longer exposure revealed that β-Trcpmyc also coprecipitated endogenous β-catenin (data not shown). (B) Immunoprecipitation of β-catenin using the same embryo extracts as in A. The precipitates were examined for β-Trcpmyc (Upper) and for β-catenin (Lower). Endogenous β-catenin is seen in lanes 1 and 4. RNA injected per embryo: 1 ng each for β-catenin, β-catenin (S→A), and β-Trcpmyc. (C and D) A complex formation between β-Trcp and Axin mediated by β-catenin. (C) Immunoprecipitation of β-Trcpmyc. The extracts were from stage 9 embryos expressing β-Trcpmyc plus AxinFlag in the presence or absence of β-catenin or β-catenin (S→A). The precipitates were examined for β-catenin (Top), AxinFlag (Middle), and β-Trcpmyc (Bottom). (D) Immunoprecipitation of AxinFlag using the same extracts as in C. The precipitates were examined for β-Trcpmyc (Top), β-catenin (Middle), and AxinFlag (Bottom). RNA injected per embryo: same as in A and B except 2 ng for AxinFlag. Image published in: Liu C et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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