XB-IMG-175015
Xenbase Image ID: 175015
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Fig. 8. Detection of xBGP mRNA by RT-PCR. Total RNA was extracted from whole froglet (65) and tissues (liver, heart, bone, kidney and muscle) of Xenopus and used to amplify xBGP mRNA by RT-PCR, using two speciï¬c primers (XBGP8F and XBGP4R), as described in Section 2. PCR products were size fractionated by agarose gel electrophoresis, transferred to a nylon membrane and hybridized with a 32P-labelled xBGP cDNA (bottom panel). To check for RNA integrity, the same RT reactions were used to amplify xODC mRNA using two speciï¬c primers based on the published sequence (top panel).
Image published in: Viegas CS et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V.
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