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XB-IMG-175473

Xenbase Image ID: 175473

Figure 3. Embryonic pibf1 expression correlates with ciliated tissues. Embryos of defined stages were analyzed for pibf1 mRNA by whole-mount in situ hybridization using a digoxigenin-labeled antisense probe. (A) Maternal transcripts in the animal hemisphere of the 4-cell embryo. (B) Expression in the involuting marginal zone tissue of the early gastrula embryo. (C) Staining of pibf1 in the axial and paraxial mesoderm at early neurula stages. (D) Pibf1 signals in skin MCCs and axial/paraxial mesoderm. (E) Expression in the otic vesicle at stage 25. (F–H) Expression pattern in tadpoles, including the head region, somites, and nephrostomes. Histological section of the eye (H′) revealed signals in the retina as well as the inner nuclear cell layer. Planes of histological sections in (B′,D′,E′,F′,H′) are indicated in the respective panels. an, animal; a, anterior; d, dorsal; DL, dorsal lip; INL, inner nuclear layer; l, left; neph, nephrostomes; no, notochord; nt, neural tube; ov, otic vesicle; p, posterior; r, right; re, retina; som, somite; v, ventral; veg, vegetal.

Image published in: Ott T et al. (2019)

Copyright © 2019 Ott, Kaufmann, Granzow, Hinderhofer, Bartram, Theiß, Seitz, Paramasivam, Schulz, Moog, Blum and Evers. Creative Commons Attribution license

GeneSynonymsSpeciesStage(s)Tissue
pibf1.LX. laevisThroughout NF stage 10involuting marginal zone
pibf1.LX. laevisThroughout NF stage 15axial mesoderm
paraxial mesoderm
pibf1.LX. laevisThroughout NF stage 20notochord
somite
multiciliated epidermal cell
pibf1.LX. laevisThroughout NF stage 25otic vesicle
pronephric mesenchyme
pibf1.LX. laevisThroughout NF stage 29 and 30head region
pronephric nephrostome
notochord
somite
spinal cord
brain
pibf1.LX. laevisThroughout NF stage 3 (4-cell)animal hemisphere
pibf1.LX. laevisThroughout NF stage 37 and 38head region
spinal cord
brain
pibf1.LX. laevisThroughout NF stage 45retinal inner nuclear layer
retina
head region

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