Xenbase Image ID: 176611

Figure S1: (a) In situ hybridization confirms the presence of Gα13 and Ric8A transcripts in migrating cranial NC cells at stage 26, as previously reported in Maldonado-Agurto et al. (2011) and Fuentealba et al. (2016). (b) Gα13 gain and loss of function controls. Left panel: Anti-Myc Western blot in embryos injected with a myc-Gα13 mRNA. Right panel: Anti-Gα13 Western blot showing that morphant embryos display diminished levels of endogenous Gα13 protein. GAPDH and α-tubulin were used as loading controls. (c) The rescue assay of the Ric-8A morpholino was performed using a myc-Ric-8A mRNA, as previously reported in Fuentealba et al.(2013). Snail2 was used as a migratory cranial NC marker at stage 23 (left panel). Snail2 expression is unaffected in stage 16 Gα13 morphant embryos (right panel). Legend: Is, injected side; Nis: non-injected side. All experiments shown here were performed in X. tropicalis. Image published in: Toro-Tapia G et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue snai2 slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. tropicalis Throughout NF stage 16 neural crest snai2 slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. tropicalis Throughout NF stage 23 cranial neural crest migratory neural crest cell gna13 G alpha 13 X. tropicalis Throughout NF stage 26 cranial neural crest brain optic vesicle ric8a LOC108696733, ric-8, ric-8A, ric8a-a, ric8a-b, synembryn, xtric-8 X. tropicalis Throughout NF stage 26 cranial neural crest brain optic vesicle Image source: Published Larger Image Printer Friendly View

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