XB-IMG-191545
Xenbase Image ID: 191545
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Fig. 4. Tp53rk expression during Xenopus development. Spatio-temporal expression pattern of tp53rk visualized by whole mount in situ hybridization (WMISH). Embryonic stages and scale bars are indicated in each panel. Upper part: WMISH with exterior view. Scale bars are equivalent to 500 m. Black dashed lines represent section planes. Lower part: WMISH following sections. Scale bars are equivalent to 100 m. (A) Animal view, (B) lateral view and (C) vegetal view of Xenopus embryos in very early stages showed a tp53rk expression in the animal pole (arrowhead) and in the developing neural plate (arrow) beside the blastopore (bp). During Xenopus embryogenesis tp53rk is strongly expressed in neural tissue which is revealed by dorsal view (D, G) in the dorsal neural tissue and neural tube (nt) and by anterior view (E, F) in the anterior neural plate (anp), neural tube (nt) and the developing eye, more precisely at the eye vesicle (ev). (H, I, J, K) Lateral view, tp53rk expression is detected in the somites (s), the eye vesicle (ev) or eye (e), the embryonic kidney (pronephric anlage (pa), pronephros (p)), the otic vesicle (ov), the brain (b) and the mandibular (ma), hyoid (ha) and branchial arch (ba) which will develop into the cranial cartilage. (L) Parasagittal view showed an expression of tp53rk in the neuroectoderm (ne). The transversal view (M, P) revealed tp53rk transcripts in the mesencephalon (m), neural crest cells (ncc) and eye vesicle (ev). Horizontal sections (N, O, Q, R) showed a strong expression in the neural crest cells (ncc), mesencephalon (m), the mandibular (ma), hyoid (ha) and branchial arches (ba), embryonic kidney (pronephric tubules (pt)) as well as in the eye, more detailed in the lens (le) and ciliary marginal zone (cmz). Image published in: Treimer E et al. (2021) Copyright © 2021. Image reproduced with permission of the Publisher, Elsevier B. V.
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