Xenbase Image ID: 26142

Fig. 3. Rx-L function is needed after eye field induction. (A, B) WMISH analysis of staged embryos injected with MoRx-L at 4-cell stage into one of the dorsoanimal blastomeres. (Aa–Af) Frontal views, injected side to the right. At NF stage 15/16, the expressions of Pax6 (Aa), Rx1 (Ab) and Six3 (Ac) are not significantly affected due to suppression of Rx-L function. (Ad–Af) At tailbud stage (NF stage 24), the expressions of Pax6 (Ad), Rx1 (Ae) and Six3 (Af) are all reduced in the injected sides. (Ba–f') Transversal gelatin/albumin sections of injected embryos probed with the markers of different retinal cell types, with dorsal side upward. The staining of each probe in the retina of injected side (Ba'–f') should be compared with that in the corresponding control side of the same embryo (Ba–f) respectively. The red dashes in Ba' mark the presumptive RPE. Suppression of Rx-L function led to a dramatically reduced expression of the photoreceptor markers, Rhodopsin (Ba, Ba') and Arrestin-C (Bb, Bb'), and a broadened expression of the bipolar cell maker, Vsx1 (Bd, Bd') and the amacrine/ganglion cell marker, Pax6 (Be, Be'). The expression levels Prox1 (Bc, Bc') and Brn3.0 (Bf, Bf') remain almost unchanged upon MoRx-L injection. (C) Effects of MoRx-L microinjection on apoptosis and cell proliferation. (Ca, b, d, e) Transversal sections (dorsal sides up and injected sides to the right) of embryos injected with 2.5pmol of MoRx-L (Ca, d) or Contr-Mo (Cb, e) subjected to TUNEL assay (Ca, b) or immunostaining of phosphorylated histone H3 (p-Histone 3) (Cd, e) at NF stage 31/32 to detect apoptotic or proliferating cells, respectively. (Cc, f) Graphs showing the numbers of TUNEL positive cells (Cc) or p-Histone 3 positive cells (Cf) in total or eye area of the non-injected side (green bars), Contr-Mo injected side (green bars), and MoRx-L (orange bars). The average of TUNEL or p-Histone-3 positive cell numbers on per section was determined in each embryo. In the TUNEL assay, for non-injected, n = 5 embryos; for Contr-Mo, n = 2 embryos; for MoRx-L, n = 3 embyros. In the p-Histone 3 detection, for non-injected, n = 5 embryos; for Contr-Mo, n = 2 embryos; for MoRx-L, n = 3 embyros. Values are given as means ± S.E.M. p = 0.28, , p < 0.05, compared with the non-injected side (Student's t-test). Quantification of the counted cells and sections is shown next to the graphs respectively. Image published in: Wu HY et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue six3 hpe2, six3-a, six3-b, XSix3, xsix3.1, xsix3.2 Xenopus Throughout NF stage 15 retina eye eye primordium optic vesicle pre-chordal neural plate pax6 an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 Xenopus Throughout NF stage 15 retina eye optic vesicle anterior neural fold neural tube rax rax1, rax-a, rax-b, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A Xenopus Throughout NF stage 15 retina eye optic vesicle pre-chordal neural plate eye primordium pax6 an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 Xenopus Throughout NF stage 24 retina eye eye primordium optic vesicle lens hindbrain spinal cord diencephalon rax rax1, rax-a, rax-b, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A Xenopus Throughout NF stage 24 retina eye eye primordium optic vesicle six3 hpe2, six3-a, six3-b, XSix3, xsix3.1, xsix3.2 Xenopus Throughout NF stage 24 retina eye eye primordium optic vesicle lens arrb2 arb2, arr2, arrestin, arrestin 2, barr2, betaarr2, beta-Arrestin 2 Xenopus Throughout NF stage 35 and 36 retina eye photoreceptor layer dorsal rho opn2, rh1, rhodopsin, rp4, xrho Xenopus Throughout NF stage 35 and 36 retina eye photoreceptor layer vsx1 ktcn, ppcd, ppd, rinx, Xvsx1 Xenopus Throughout NF stage 37 and 38 retina eye retinal inner nuclear layer pou4f1.L Brn3.0, brn3a, LOC108709765, oct-t1, POU class IV homeobox 1, rdc-1 X. laevis Throughout NF stage 37 and 38 retina eye retinal ganglion cell layer prox1 prospero, prospero homeobox protein 1, prospero-related homeobox 1, Prox 1, xprox1 Xenopus Throughout NF stage 37 and 38 retina eye lens retinal inner nuclear layer pax6 an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 Xenopus Throughout NF stage 37 and 38 retina eye lens retinal inner nuclear layer Image source: Published Larger Image Printer Friendly View

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