Xenbase Image ID: 40628

Figure 2. Insm1 is required for endocrine cell differentiation. A-D: Insulin expression at both early (NF35; 90%, n = 15) and late stages (NF40/42; 79%, n = 52) is almost completely abolished. E-H: glucagon expression. I-L: somatostatin expression. At early stages expression of both gcg and som is reduced in the stomach/duodenum of insm1 knockdown animals (100%; n = 33 and n = 12, respectively), but begins to reappear at later stages (100%; n = 8 for both). The initial expression of these markers in the pancreas is also reduced at later stages (100% for gcg and 80% for som). M,N: Expression of the exocrine pancreas marker XPDIp is normal in insm1 morpholino injected tadpoles (100%, n = 10). O: In vitro transcription and translation of insm1 either alone (insm1), in the presence of the 5-bp mismatch morpholino (insm1+mMO) or in the presence of the morpholino (insm1+MO). Pictures presented in control are of uninjected embryos, but in all cases mismatch morpholino embryos (n ge 8 for each marker) gave identical results as control, with normal expression of these markers. The pancreas is outlined. Image published in: Horb LD et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue ins ins1, ins-a, ins-b, insulin, insulin I, preproinsulin, xins X. tropicalis Throughout NF stage 35 and 36 endoderm dorsal gcg glucagon, proglucagon I X. tropicalis Throughout NF stage 35 and 36 stomach duodenum sst smst, som, somatostatin, sst.1 X. tropicalis Throughout NF stage 35 and 36 stomach duodenum pdia2 pdi, pdip, XPDIp X. tropicalis Throughout NF stage 35 and 36 pancreas gcg glucagon, proglucagon I X. tropicalis Throughout NF stage 42 pancreas stomach duodenum sst smst, som, somatostatin, sst.1 X. tropicalis Throughout NF stage 42 pancreas stomach duodenum ins ins1, ins-a, ins-b, insulin, insulin I, preproinsulin, xins X. tropicalis Throughout NF stage 42 pancreas Image source: Published Larger Image Printer Friendly View

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