Xenbase Image ID: 75394

Fig. 3. Temporal and spatial expression of elrD transcripts. (A) One g of total RNA was reverse-transcribed and PCR performed by incorpo- rating digoxigenin-dUTP. PCR products were resolved by electrophore- sis in a 1% agarose gel and detected as described in Materials and Methods. Sequence analysis of the cloned products has shown that bands 1 and 2 correspond to elrD1 and elrD2 transcripts, respectively. Band 3 corresponds to an incomplete elongation of elrD1 and elrD2 transcripts. N: Neurula, T: Tailbud, B: Adult Brain. (B,C) In situ hybridiza- tion at tailbud stage with an antisense elrD1 (B) and elrD2 (C) probes. expression is seen in the brain vesicles (arrowheads), the cranial ganglia (arrows) and the spinal cord (block arrow). Image published in: Nassar F (2011) Copyright © 2011. Image reproduced with permission of the Publisher, University of the Basque Country Press. Gene Synonyms Species Stage(s) Tissue elavl4.L elrD, elrD1, elrD2, HuD, LOC108715589 X. laevis Throughout NF stage 32 nervous system brain spinal cord eye forebrain telencephalon midbrain hindbrain cephalic nerve olfactory bulb trigeminal nerve facial nerve glossopharyngeal nerve vagus nerve Image source: Published Larger Image Printer Friendly View

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