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Fig. 6. mRNA expression of GPAT1 and GPAT2 in different organs detected by whole- mount in situ hybridization. (A) GPAT1 is expressed during kidney and liver organogenesis. Whole-mount in situ hybridization with the GPAT1 antisense riboprobe of embryos at the indicated stages is shown in the left column. Higher magnifications of the kidney and liver regions are shown in a lateral view in the right two panels (anterior to left). Eye (E), kidney (K) (early expression detected at stage 26) and liver (L) (early expression detected at stage 33/34) are shown. (B) GPAT1 and GPAT2 show a different expression pattern at stage 40. GPAT1 and GPAT2 are both expressed in the nervous system (E, eye; B, brain; and SC, spinal cord). Further, GPAT1 is strongly expressed in liver (L) and kidney (K), whereas GPAT2 is expressed in presumptive gonad (testis-T) and weakly in kidney. Image published in: Bertolesi GE et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue gpam.S gpat, gpat1 X. laevis Throughout NF stage 26 eye pronephric mesenchyme head region spinal cord brain gpam.S gpat, gpat1 X. laevis Throughout NF stage 28 eye forebrain hindbrain otic vesicle pronephric mesenchyme intersomitic region head region cranial neural crest gpam.S gpat, gpat1 X. laevis Throughout NF stage 32 spinal cord eye otic vesicle pronephric kidney pronephric duct head region pharyngeal arch brain gpam.S gpat, gpat1 X. laevis Throughout NF stage 33 and 34 spinal cord eye otic vesicle pronephric kidney pronephric tubule pharyngeal arch liver primordium brain gpam.S gpat, gpat1 X. laevis Throughout NF stage 37 and 38 head region spinal cord eye otic vesicle pronephric kidney pronephric tubule liver primordium pharyngeal arch gpam.S gpat, gpat1 X. laevis Throughout NF stage 40 head region spinal cord eye forebrain hindbrain otic vesicle pronephric kidney liver gpat2 X. tropicalis Throughout NF stage 40 head region spinal cord eye brain otic vesicle germ cell pronephric kidney Image source: Published Larger Image Printer Friendly View

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