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Fig. 2. Expression pattern of XTgfbi. (A) RT-PCR shows, in stage 10 embryos, XTgfbi was strongly expressed in DMZ and weakly expressed in VMZ and animal cap. Gsc and Xwnt-8 are markers for DMZ and VMZ, respectively. (B) Whole mount in situ hybridization of XTgfbi. Dashed lines indicate the position of transversal sections as labelled. (B) Dorsal view (rostral to the left) at stage 14. XTgfbi was weakly expressed in the dorsal midline. (C) Dorsal view at stage 16 showing a decreasing XTgfbi expression gradient in the dorsal midline. (D, D') Lateral and dorsal views at stage 22: XTgfbi expression was seen in the dorsal tips of developing somites. (E, F) Lateral and dorsal views at stage 25: XTgfbi was expressed in dorsal somite tips (arrowheads), weakly in tissues surrounding the eye (arrow). (G) Lateral view at stage 28: XTgfbi was expressed in intersomitic boundaries, around the eye, migrating cranial neural crests and tailbud. (H) Lateral view of embryo head at stage 33: XTgfbi expression was prominent in CNS (arrowhead), eye (arrow) and pitpharyngeal pouches (asterisk). (I) Side view at stage 33 showing strong expression in the somites, tailbud and migrating hypaxial muscle anlagen (arrowheads). (J) Lateral view at stage-36: XTgfbi expression is prominent in the migrating hypaxial muscle (arrowheads), eye and tail fin. (K) Lateral view at stage 39: XTgfbi is detected in migrating hypaxial muscle and tail fin (arrows). (L) Lateral view at stage 42: XTgfbi is expressed in ventral side of the embryo and migrating hypaxial muscle (arrowheads). (M, N, O) In situ hybridization with sense XTgfbi probe shows no specific staining. (P, Q) Transversal sections from C: XTgfbi was strongly expressed in the floor plateand notochord. (R, S, T) Transversal sections from G: XTgfbi was expressed in neural crest cells and somites. (U, V) Parasagittal sections from G: XTgfbi expression is in the somites, strong in the dorsal tips (asterisk) and intersomitic boundaries (arrowheads). (W) Transversal sections from I: XTgfbi expression is in olfactory pits, periphery of prosencephalon, migrating neural crests, pharyngeal pouches as well as rhombencephalon, otic vesicles and notochord. (Z) Sagittal section from L showing XTgfbi expression in tissues surrounding various internal organs and abdominal epithelia. fp, floor plate; int, intestine; liv, liver; mes, mesenchyme; nc, notochord; ncc, neural crest cell; olf, olfactory pit; ov, otic vesicle; pha, pharygneal pouch; rho, rhombencephalon; som, somite; stm, stomach. Scale bar=100 m. Image published in: Wang F et al. (2013) Copyright © 2013. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tgfbi.L betaig-h3, bigh3, cdb1, cdg2, cdgg1, csd, csd1, csd2, csd3, ebmd, keratoepithelin, lcd1, LOC108712407, xtgfbi X. laevis Throughout NF stage 14 to NF stage 16 notochord floor plate tgfbi.L betaig-h3, bigh3, cdb1, cdg2, cdgg1, csd, csd1, csd2, csd3, ebmd, keratoepithelin, lcd1, LOC108712407, xtgfbi X. laevis Throughout NF stage 22 to NF stage 25 somite periocular region tgfbi.L betaig-h3, bigh3, cdb1, cdg2, cdgg1, csd, csd1, csd2, csd3, ebmd, keratoepithelin, lcd1, LOC108712407, xtgfbi X. laevis Throughout NF stage 28 somite tail fin presomitic mesoderm tail bud intersomitic region migratory neural crest cell periocular region cranial neural crest tgfbi.L betaig-h3, bigh3, cdb1, cdg2, cdgg1, csd, csd1, csd2, csd3, ebmd, keratoepithelin, lcd1, LOC108712407, xtgfbi X. laevis Throughout NF stage 33 and 34 somite hypaxial muscle hindbrain notochord floor plate neural crest visceral pouch olfactory pit migratory neural crest cell periocular region intersomitic region tail bud otic vesicle tgfbi.L betaig-h3, bigh3, cdb1, cdg2, cdgg1, csd, csd1, csd2, csd3, ebmd, keratoepithelin, lcd1, LOC108712407, xtgfbi X. laevis Throughout NF stage 35 and 36 eye hypaxial muscle tail bud tail fin olfactory region intersomitic region posterior tgfbi.L betaig-h3, bigh3, cdb1, cdg2, cdgg1, csd, csd1, csd2, csd3, ebmd, keratoepithelin, lcd1, LOC108712407, xtgfbi X. laevis Throughout NF stage 39 hypaxial muscle tail fin intersomitic region olfactory region stomodeal-hypophyseal primordium tgfbi.L betaig-h3, bigh3, cdb1, cdg2, cdgg1, csd, csd1, csd2, csd3, ebmd, keratoepithelin, lcd1, LOC108712407, xtgfbi X. laevis Throughout NF stage 42 hypaxial muscle tail fin ventral epithelium stomach intestine liver Image source: Published Larger Image Printer Friendly View

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