Xenbase Image ID: 152378

Fig. 6. C-cadherin localization on lamellipodia depends on RLC. Actin is red and C-cadherin or caC-cadherin is green. (A,B) Cadherin forms puncta (arrow) on lamellipodia (A) that resolve into long plaques (arrow), linking actin cables from neighboring cells (B, asterisk). (C) By contrast, lamellipodia on RLC morphant cells maintain a cloud of diffuse C-cadherin signal (arrow) that does not resolve into puncta. (D) Time-lapse microscopy of C-cadherin dynamics in adjacent control cells labeled singly for actin (left) or C-cadherin (right) display adhesions (arrows) that when broken, snap backwards rapidly (asterisk). (E) RLC MO-treated cells (co-injected with a blue dextran) do not make adhesions with neighboring control cells. (F) pnRLC-expressing morphant cells display little protrusive activity or adhesion remodeling (arrow). (G-I) Cells expressing caC-cadherin exhibit longer linear adhesion plaques than C-cadherin-expressing cells (arrows in G,H), which are associated with thicker actin cables (arrow in G′, quantified in I; n=20 widths per condition). Merged images are in G′,H′. Scale bar in A is 20†μm for all images. Error bars represent s.e.m. ***P<0.001. Image published in: Pfister K et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue myh10.L LOC108701185, nmmhcb, non-muscle myosin, nonmuscle myosin X. laevis Sometime during NF stage 1 to NF stage 66 cell actb.L beta actin, beta-actin, g-actin X. laevis Sometime during NF stage 1 to NF stage 66 cell cdh3.L b-cadherin, c-cadherin, cdh3-a, cdh3-b, cdhc-A, cdhp, EP-cadherin, hjmd, pcad, P-cadherin, U-cadherin, xb-cad, XBcad, XB-cadherin X. laevis Sometime during NF stage 1 to NF stage 66 cell Image source: Published Larger Image Printer Friendly View

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