Xenbase Image ID: 77993

Figure 1. A newly identified allele of AXPC is predominantly expressed during early development. A: Comparison of the sequence of the two alleles of AXPC surrounding the ATG start codon. MO-1 refers to a previously published construct (orange). MO-2 is a newly designed morpholino (green). Red box indicates the ATG start codon. *Differences between the sequences. B: Western blot detection of both exogenous and endogenous expression of AXPC in lysates from embryos collected at the indicated stage. Embryos over-expressing AXPC (lane: AXPC) were collected at stage 12.5. C: Western blot of endogenous AXPC in embryo lysates at stage 12.5 injected with the indicated amount of control morpholino or AXPC morpholino. For co-injections, morpholino-1 and morpholino-2 were mixed in equal parts to give the indicated final amount. In B and C, AXPC was detected by Western blotting with anti-AXPC antibody. Each lane represents the equivalent of 1 embryo.Download figure to PowerPoint Image published in: Yoder MD and Gumbiner BM (2011) Copyright © 2011. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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