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XB-PHENO-25324

Xla Wt + wnt3a + chd1 + animal cap explant, Wang H et al. (2021)

Experiment + Assay: Xla Wt + wnt3a + chd1 + animal cap explant + NF15 (RT-PCR)
Background: Xla.pigmentedNasco
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection chd1.L chd1.L
 NF stage 2 (2-cell) blastomere
mRNA injection wnt3a.L wnt3a.L
 NF stage 2 (2-cell) blastomere
explant  
NF stage 8 animal cap
Conditions:
Assay Type Assay Stages Antibody
RT-PCR NF stage 15  
Expression Phenotypes (3):
increased amount foxd3.L expression in the animal cap
increased amount snai2.L expression in the animal cap
increased amount sox10.L expression in the animal cap
Source: Fig.3.B
Article: XB-ART-58113, PubMed