XB-PHENO-25631
Xla Wt + pnhd-FLAG + animal cap explant, Ossipova O et al. (2020)
| Experiment + Assay: |
Xla Wt + pnhd-FLAG + animal cap explant +
NF10.5-11 (RT-PCR)
|
| Manipulations: |
| Manipulation Type |
Reagent |
Target Gene |
Stage |
Treatment Area |
|
mRNA injection
|
Tg(Xla.pnhd.L-FLAG)
|
pnhd (Xla)
|
NF stage 4 (8-cell)
|
animal blastomere
|
|
explant
|
|
|
NF stage 10
|
animal cap
|
|
| Conditions: |
| Assay Type |
Assay Stages |
Antibody |
|
RT-PCR
|
NF stage 10.5
to NF stage 11
|
|
|
|
|
Expression Phenotypes (5):
|
|
increased amount
cdx4.L
expression in the animal cap
|
|
increased amount
fgf8.L
expression in animal cap
|
|
increased amount
hoxd1.L
expression in the animal cap
|
|
increased amount
tbxt.S
expression in the animal cap
|
|
increased amount
wnt8a.L
expression in the animal cap
|
|
