XB-PHENO-26010
Xtr wt + thra TALEN + mCherry, Nakajima K et al. (2018)
| Experiment + Assay: |
Xtr wt + thra TALEN + mCherry +
NF50-58 (whole-mount microscopy)
|
| Manipulations: |
| Manipulation Type |
Reagent |
Target Gene |
Stage |
Treatment Area |
|
TALEN
|
TALEN-ELD/KKR mRNA mixture was injected into one side of two- or four-cell embryos with mCherry mRNA to modify TRα or TRβ.
|
thra
|
NF stage 2 (2-cell)
|
left side
|
|
lineage tracer
|
mCherry
|
|
NF stage 2 (2-cell)
|
left side
|
|
| Conditions: |
| Assay Type |
Assay Stages |
Antibody |
|
whole-mount microscopy
|
NF stage 50
to NF stage 58
|
|
|
|
