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Figure 3. Voltage-clamp of oocytes injected with different combinations of the four O. dentatum nAChR subunits.(A) Depiction of a possible arrangement of O. dentatum UNC-29, UNC-63 & UNC-38. ‘X’ represents any of the subunits. PyR, pyrantel; Tbd, tribendimidine, ACh, acetylcholine; Nic, nicotine; Bep, bephenium; The, thenium. (B) Representative traces of inward currents elicited by the various agonists in oocytes injected with 1∶1∶1 Ode-unc-29∶Ode-unc-63∶Ode-unc-38. Pyr & Tbd were the most potent agonists on this receptor subtype. (C) Bar chart (mean ± se) of currents elicited by the different agonists in the Ode-(29 - 38 - 63) Pyr/Tbd-nAChR (paired t-test, **p<0.01, ***p<0.001). (D) Dose-response of normalized currents vs log concentration for Pyr (inverted Δ, n = 6), Tbd (▴, n = 6) and ACh (•, n = 5) in the Pyr/Tbd-nAChR. (E) Diagrammatic representation of the three subunits, unc-29, unc-63 and acr-8 injected into oocytes. ‘X’ could be any of the three subunits. (F) Representative traces of inward currents produced by the different agonists in oocytes injected with 1∶1∶1 Ode-unc-29∶Ode-unc-63∶Ode-acr-8. (G) Bar chart (mean ± se) of normalized currents elicited by the different agonists in the Ode(29-63-8) receptor subtype. All currents normalized to 100 µM ACh currents; comparisons made with the ACh currents (paired t-test, *p<0.05, **p<0.01, ***p<0.001). (H) Dose-response plot of normalized currents vs log. Concentration of Pyr (inverted Δ, n = 6), Tbd (▴, n = 6), ACh (•, n = 6) and Lev (▪, n = 6). (I) Representation of a possible arrangement of the four O. dentatum subunits injected into Xenopus oocytes. (J) Representative traces of inward currents elicited by the different agonists on the Ode(29–63–38–8) or Lev-nAChR. (K) Bar chart (mean ± se) of normalized currents elicited by the different agonists on the Lev-nAChR subtype. Comparisons were made with 100 µM ACh currents, which was used for the normalization (paired t-test, ***p<0.001). (L) Dose-response plot of normalized currents against log of Tbd (▴, n = 6), ACh (•, n = 18) and Lev (○, n = 5) concentrations. Image published in: Buxton SK et al. (2014) Image reproduced on Xenbase with permission of the publisher and the copyright holder. Creative Commons Attribution license Larger Image Printer Friendly View

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