XB-IMG-133143
Xenbase Image ID: 133143
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Fig. 2. Expression pattern of
TSC-22 (a) Temporal expression
pattern of XTSC-22 mRNA detected
by reverse transcriptionpolymerase
chain reaction (RTPCR).
Transcripts were first
detected at late blastula (stage 9)
and persisted until tadpole stage
(stage 35). Ornitine decarboxylase
(ODC) expression serves as
the quantitative control. RT-PCR
was carried out to avoid contamination
with genomic DNA. (bâf)
Spatial expression pattern of
XTSC-22 determined by wholemount
in situ hybridization. At
stage 10 (b,c), the ectoderm was
stained. (c) Sagittal section of the
stage-10 embryo in (b). The black
lines indicate the blastopore. At
neurula stage (d,e), transcripts
were observed in the neural
region including eyes and brain.
This localization was clear at
stage 35 (f). Bars, 0.3 mm (g)
Induction of XTSC-22 transcription
by growth factors: there was
no change in XTSC-22 expression
with any treatment. WE,
whole embryo. Image published in: Hashiguchi A et al. (2004) Copyright © 2004. Image reproduced with permission of the Publisher, John Wiley & Sons.
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