Xenbase Image ID: 156434

Fig. 1. Homez structure and gene expression during embryogenesis. (A) Shematic representation of the Homez protein. HD, homeodomain; L, leucine repeats, A, acidic domain. (B) Temporal expression of Homez by RT-PCR. RNA was extracted from embryos at the indicated stages. (C) RT-PCR analysis of Homez expression in dissected explants of stage 10.5 embryos. The endodermal VegT, mesodermal Xbra and ectodermal Xema markers were used as dissection controls. In B and C, Histone H4 was used as a loading control. -RT, control RT-PCR without reverse transcriptase. (D-I) Spatial expression of Homez compared to that of Ngnr1, Myt1 and N-tubulin analyzed by whole-mount in situ hybridization. Nieuwkoop-Faber stages are indicated. (D–H) Dorsal views. (I) Lateral view. Stripes of Homez expression in the domains of primary neurogenesis in the posterior neural plate are indicated (l, lateral; i intermediate; m, medial). Note that Homez is activated in the domains of primary neurogenesis at E12.5 when N-tubulin is not yet detectable. (J–M) Homez expression compared to that of Ngnr1, Myt1 and N-tubulin in the neural tube of stage 32 embryos. Sections at the level of the otic vesicle are shown. Note that XHomez+ cells are detected more medially than N-tubulin in the marginal zone. Abbreviations: AC, animal caps; ba, branchial arches; cg, cranial ganglia; e, egg; CE, control embryo; MZ, marginal zone; VP, vegetal pole. Image published in: Ghimouz R et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue neurog1.L neurod3, neurogenin, neurogenin 1, neurogenin-1, neurogenin1, ngn, ngn1 X. laevis Throughout NF stage 12.5 neuroectoderm myt1.L X-MyT1, xmyt1, XMyTI X. laevis Throughout NF stage 12.5 neuroectoderm homez.L X. laevis Throughout NF stage 12.5 neural plate tubb2b.L NBT, neural beta-tubulin, NST, N-Tub, n-tubulin, ntubulin, Xn-tubulin X. laevis Throughout NF stage 12.5 homez.L X. laevis Throughout NF stage 14 neural plate homez.L X. laevis Throughout NF stage 28 brain hindbrain midbrain forebrain central nervous system eye cranial nerve pharyngeal arch cranial ganglion homez.L X. laevis Throughout NF stage 32 neural tube otic vesicle neurog2.L atoh4, neurog2-a, neurog2-b, neurogenin 2, neurogenin-2, neurogenin2, neurogenin-related-1, ngn-2, ngn2, ngnr, Ngnr-1, Ngnr1, ngnr 1a, xngn2, X-ngnr-1, Xngnr-1, XNgnr1 X. laevis Throughout NF stage 32 neural tube myt1.L X-MyT1, xmyt1, XMyTI X. laevis Throughout NF stage 32 neural tube tubb2b.L NBT, neural beta-tubulin, NST, N-Tub, n-tubulin, ntubulin, Xn-tubulin X. laevis Throughout NF stage 32 neural tube Image source: Published Larger Image Printer Friendly View

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