XB-IMG-43351
Xenbase Image ID: 43351
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Fig. 7. Effects of Cdx knockdown during post-gastrula stages. All samples are Xenopus tropicalis except (FâH) which are Xenopus laevis explants, which were used because their bigger size allowed easier of processing for in situ hybridization. A: Expression of the trunk/posterior mesodermal markers MyoD and brachyury (Xbra) is unaffected in Cdx knockdown embryos at late neurula stage 20. Embryos were injected unilaterally with 10 ng of combined Cdx1, Cdx2, and Cdx4 morpholino oligos (MOs); red asterisk indicates injected side. Left and middle panels are dorsal views with anterior to the left; right panel is a posterior view with dorsal to the top. B: Expression of the ventral mesodermal markers Scl and Vent2 is down-regulated in Cdx MO knockdown embryos at stage 20. Embryos were injected bilaterally (Scl) or unilaterally (Vent2) with 10 ng per blastomere of the triple Cdx MO combination at the two-cell stage. Left panels are ventral views with anterior to the left; middle panels are dorsal views with anterior to the left and right panels are posterior views with dorsal to the top. Red asterisk indicates the injected side. C: Wnt11 expression is unaffected in triple Cdx knockdown embryos (40-ng total dose) at the early neurula stage 14. Top panels are dorsal views with anterior to the left, and bottom panels are ventral views with anterior to the left. D: Posterior expression of Wnt5A and Wnt3A is down-regulated in triple Cdx knockdown embryos (40-ng total dose) at early neurula stage 14. Dorsal and posterior views are indicated. Dorsal views with anterior to the left and posterior views with dorsal to the top. Black arrows indicate posterior paraxial mesoderm. E: Increasing Cdx activity in embryos up-regulates Wnt3A and Wn5A expression. Embryos were injected with15 pg Xcad-VP16 mRNA. Dorsal views anterior to the left. F: Increasing Cdx function in animal caps up-regulates Wnt3A and Wnt5A expression. Animal cap explants from embryos injected with 50 pg of Xcad-VP16 mRNA were cultured until early neurula stage 14. G: Wnt3A expression is upregulated in animal caps from embryos injected with 250 pg of Cdx1 mRNA. (H) Cdx1 expression is up-regulated in animal caps from embryos injected with 50 pg of Wnt3A mRNA. Image published in: Faas L and Isaacs HV (2009) Copyright © 2009 Wiley-Liss, Inc. Creative Commons Attribution license Larger Image Printer Friendly View |